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Moreover, computational modeling shows that stem cell arrangement and positioning of axillary meristems distribute somatic mutations around the main shoot, preventing their fixation and maximizing genetic heterogeneity.
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Auxin moves down the main shoot of the plant to prevent new branches from forming.
Shoot samples (PO:0009006; shoot system, PO:0007112; 1 main shoot growth stage, Cooper et al. 2013) were collected at 5 days after nutrient starvation.
The main shoot branches at least 4 times.
The main shoot is slightly curved, with nodes and internodes.
Branch order for each inflorescence-bearing shoot was determined by careful visual inspection, with the main shoot being 1st-order, lateral shoots emerging directly from the main shoot being 2nd-order, lateral shoots emerging from 2nd-order shoots being 3rd-order, and so forth (Fig. 1b, bottom right panel).
Pollination was done by vibrating the main shoot two times per week.
The main shoot bears internodes and swollen nodes, from which lateral branches arise oppositely.
The health of lateral branches was used as a major criterion of main shoot health because these branches senesce as shoots age (Fig. 2A).
Systemic colonization was determined in apical segments of the main shoot at the onset of silique maturity.
Plants were 0.90 1.70-m high and had one clearly dominant main shoot and twigs of maximally the second order.
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