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Auxin moves down the main shoot of the plant to prevent new branches from forming.
In experiment E3, apical pieces of the main shoot of parents, F1 and F3-families were also analysed for the amount of fungal DNA in the plant tissue by quantitative real-time PCR.
Later, during early spring after leaf emergence (mid-May), the uppermost 15 cm of the main shoot of each plant in a different sub-block was defoliated and the apical bud was removed, to simulate leaf stripping by a browser, for example moose.
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During late winter earlyy March) the main shoot on each of one sub-block of plants was cut 10 cm above ground before leaf emergence, to simulate winter foraging by voles or hares.
Maximum stomatal conductances of water vapour (gw) and transpiration of main shoots of giant horsetails were measured using a Li-Cor LI-1600 steady-state porometer (Li-Cor Biosciences, Lincoln, NE, USA) by sealing the chamber vertically against an internode in the mid-section of young but mature and healthy shoots with green branches at each site.
To determine systemic spread by re-isolation, segments of approx. 3 cm length were cut from the apex of the main shoot axis of each plant, surface-sterilised with 0.1% (w/v) sodium hypochlorite solution and subsequently dipped in 70% (v/v) ethanol.
Third, the auxin response reporter construct DR5::GUS [ 61] has increased activity in the main shoot axis of max2-1 plants [ 60], associated with increased amounts of auxin moving in the PATS [ 44].
At anthesis, 65 70 identical main shoot spikes of each cultivar from every experimental units were ear marked.
Systemic colonization was determined in apical segments of the main shoot at the onset of silique maturity.
Plants were 0.90 1.70-m high and had one clearly dominant main shoot and twigs of maximally the second order.
A key aspect of the adaptation of landraces was the small size of the main shoot panicle, as it minimised (1) the loss of productive tillers during stem elongation; (2) the delay in anthesis if mid-season drought occurs; and (3) the reduction in panicle productivity of the basal tillers under stress.
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