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In six-year-old roots, the transcripts were strongly expressed in rhizomes, followed by lateral roots and main root bodies.
Only transcripts encoding 3-hydroxy-3-methylglutaryl-coenzymeA reductase (HMGR), phosphomevalonate kinase (PMK), and geranylgeranyl diphosphate synthase (GPS) were highly expressed in main root bodies.
In particular, transcripts encoding MT and DRM1 were the most abundant in main root bodies, lateral roots, and rhizomes of six-year-old roots.
Transcripts encoding HMGR and 1-deoxy-D-xylulose-5-phosphate synthase (DXS) were also identified as DE genes between one- and six-year-old main root bodies and rhizomes.
Here, we used the Illumina platform to generate over 232 million RNA sequencing reads from four root samples, including whole roots from one-year-old plants and three types of root tissue from six-year-old plants (i.e., main root bodies, rhizomes, and lateral roots).
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The ginsenoside contents are highest in five- and six-year-old rhizomes, followed by lateral roots and the main root body [ 44– 44].
Six-year-old roots were collected from plants with fully expanded leaves grown in a ginseng experimental field (Suwon, Korea), which were divided into three parts, including the main root body, lateral roots (including fine roots), and rhizomes.
We identified three HMGR transcripts based on KEGG annotation, one of which was more highly expressed in the main root body than in lateral roots, as observed by Kim et al. [ 47].
Among these, 32 transcripts were uniquely expressed in one-year-old roots, while seven were uniquely expressed in one of three tissues of six-year-old roots, including, one, one, and five uniquely expressed transcripts in the main root body, lateral roots, and rhizomes, respectively.
There is no main root, and the plant body of many taxa is best described as like a thallus, although some species look like odd members of Apiaceae but with two-ranked leaves.
Taproot, Main root of a primary-root system.
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