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The mutations we identified fall into four main phenotypic classes.
Genes required for muscle development and function are grouped into two main phenotypic classes of mutants, Pat (paralyzed and arrested elongation at the two-fold length of embryonic development) and Unc (Uncoordinated), with some genes capable of producing both phenotypes depending on the nature of the mutation [ 10, 11].
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It is most clear from this analysis that complementary phenotypic classes are independent.
A principal component analysis revealed two main phenotypic groups, partially correlating with previously described genomic clusters.
The necessity of establishing boundaries for phenotypic classes arises because, although pigment intensity is a continuous variable, genotypes are, by their nature, discrete classes.
Under complete submergence, the main phenotypic effect observed in SUB1A-1 overexpression and Sub1 near isogenic lines (NILs), is a significantly reduced elongation growth (Additional file 1: Figure S1b).
Deleterious alleles were classified into seven phenotypic classes.
The main phenotypic variance indeed appeared as channeling evolution between populations.
Local populations were found to differ in genetic organization and frequency distribution of phenotypic classes.
Consequently, eight resulting phenotypic classes of hosts are treated in the model.
This identified 4 phenotypic classes of drug suppressors, which included known and novel genes.
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