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These mRNAs often contain a 5'-UTR considerably longer than the average cellular 5'-UTR [13], [14], may contain AUG codons upstream of the initiation codon for the main open reading frame, and have complex secondary structures [15].
Translations of the rice cDNA sequences in the same frame as the predicted main open reading frame, starting from the 5'-untranslated region to the end of the main open reading frame, were used to search (blastp) against UniProtKB.
The SaV genomes are predicted to contain either 2 or 3 main open reading frames (ORF1 3).
Moreover, mutating the last 10 sense codons of the uCC gave similar results, eliminating polyamine-induced repression of main open reading frame translation.
Also, the main open reading frames, predicted by uORFSCAN were used to search (blastn) the coding sequence (CDS) annotations from TIGR rice pseudomolecules database.
As additional verification, the rice main open reading frame predictions were also compared with protein data from The UniProt Knowledgebase (UniProtKB).
Similar(40)
The main SPG4 open reading frame (ORF) starts with an AUG that resides in a context that deviates significantly from the consensus motif (TGAaugA).
Approximately 40% of mammalian mRNA sequences contain AUG trinucleotides upstream of the main coding sequence, with a quarter of these AUGs demarcating open reading frames of 20 or more codons.
Since the sequencing gel resolves to 1 bp, but the main peaks are every 3 bp (reflecting the predominance of open reading frame rearrangements of IGH), we took the values for the peaks at 3-bp intervals for further analysis.
The open reading frame can be divided into three main regions: P1 (encoding capsid proteins VP0, VP3, VP1), P2 (nonstructural proteins) and P3 (nonstructural proteins, including the viral RNA polymerase) [ 2- 4].
Nearly all resistant transcripts possess at least one efficiently translated upstream open reading frame (uORF) that represses translation of the main coding ORF under normal conditions.
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