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No additional splice variants, specific for embryonic stages, were observed for any of the main murine B" subunits.
In contrast, five EST-clones of the specific N-terminus of mPR59/B δ3 and multiple (> 15) EST-clones of the other B -specific N- and C-termini were found, suggesting that mPR72/B -specific0/B"α1, mG5PR/B"γ, mPR59/B"δ1, PR59/B"δ2, and PR59/B"δ3 and much more abundant, and probably represent the main murine B" isoforms.
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RNA amplification correlates with cell cycle entry and cell growth in P493-6 and primary murine B cells.
At the cellular level, we have also determined the subcellular localisation of the main murine and human B" subunits via expression of EGFP-fusion proteins in COS7 cells (for PR72/B α2, G5PR/B γ, PR70/B β1, PR59/B δ1 and PR59/B δ2) or via indirect immunofluorescence (for PR130/B α1).
Primary splenic murine B cells were isolated using B-cell isolation kit (Miltenyi Biotech).
G. Expression, distribution and specificity of Fc receptors for IgM on murine B cells.
Murine B cells cultured in organoids for four days in an extracellular matrix.
Human and murine B cells and thymic epithelial cells express DO, while monocytes/macrophages do not.
We conclude that CD81 is differentially expressed in the murine immune system, and is involved in regulating the adhesion and activation of murine B cells.
Idiotype variants of 38C13, a murine B cell lymphoma, have been isolated by immunoselection with antiidiotype mAbs.
Grabstein, K. H. et al. Inhibition of murine B and T lymphopoiesis in vivo by an anti-interleukin 7 monoclonal antibody.
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