For the main culture, 0.5 mL of inoculums in the main medium was prepared in 250 mL Erlenmeyer flasks.
The main medium was 25 mL of distilled water containing 0.5 g yeast extract, 0.5 g peptone, 0.1 g MgSO4∙7H2O, and 0.1 g K2HPO4, to which 25 mL of a saccharified liquid containing 62 g/L glucose was added.
The latter was also used in D-stat experiments as the main cultivation medium, additionally the main medium was supplemented with acetic acid and prepared as follows: 300 ml medium was withdrawn from the main cultivation medium and supplemented with 3 ml of glacial acetic acid (99.9%).
In addition, a second medium was used in D-stat where the main medium was supplemented by acetic acid and prepared as follows: 300 ml medium was withdrawn from the main cultivation medium and supplemented with 3 ml of glacial acetic acid (99.9%).
Jacob El Hanani's main mediums are not tangibles, but the concepts of minuteness and time.
Maddy Young's main mediums are fairly traditional; some might even say old-fashioned.
The configuration proposed considers preheating of the air before compression which increases the fraction of the total exergy that is stored in the form of high-grade heat in comparison to existing designs in which the main exergy storage medium is the compressed air itself.
The composition of the main culture medium was adapted from Mandels & Weber [ 61], using 10 g/L of pretreated delignified sugar cane bagasse (DEB) plus SB, at a 3 1 ratio [ 25].
Especially, the magnesium-based fuel grain consisting of most magnesium, small ammonium perchlorate, and some binder is carried on-board, while water as the main oxidizer and working medium is injected into the chamber through different pipelines.
The main point is that, this medium is more economical than others, especially for the large-scale productions.
