We determined the absolute counts of the main lymphocyte populations in a large group of DS children to gain further insight into this immunodeficiency.
Flow cytometry will be performed using a standard multicolour procedure to detect the main lymphocyte populations in human blood.
Assessment of the main lymphocyte populations of the NPP workers done by us for the last 10 years didn't show significant differences in the main lymphocyte populations B and T lymphocytes and NK cells.
In our previous study on the same contingent of NPP workers, no significant differences were found between the exposed groups and the control regarding the main lymphocyte populations: B and T lymphocytes and NK cells with phenotypes CD3-16+56+.
No significant differences were found between the exposed groups and the control in terms of the main lymphocyte populations - B and T lymphocytes, NK cells with phenotypes CD3-CD16+ CD57+ and CD57+ CD57+
In addition to the main lymphocyte populations CD3+, CD19+, CD4+, and CD8+, tested in a previous study, in the present investigation CD4+25+, CD4+62L−, CD4+62L+, CD8+28+, CD8+38+ were also analyzed.
The main source of CSF antibodies are lymphocytes B, but in patients with TBE lymphocytes B comprise only 3-53-5% CSF lymphocyte population [ 12].
Results were expressed as numbers of cells per microliter of blood for lymphocyte subsets and as percentage of positive cells among total CD4+ lymphocyte population for Treg.
c CAM5.2-red immunostain is completely negative, confirming the absence of RPE (magnification, ×200) Fig. 3 Immunohistochemical staining of the ERM from case 1, demonstrating a predominance of lymphocytes among the inflammatory cell population, and out of the lymphocyte population, a predominance of T cells over B cells. a CD45-red immunostain highlights lymphocytes (magnification, ×100).
Consistent with their phenotype, sooty mangabey NKT lymphocytes were significantly enriched for CD8+ T lymphocytes and had significantly lower number of CD4+ T lymphocytes compared to the circulating total T lymphocyte population (Table 1).
