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The main image processing procedures are performed using programs from EMAN (Ludtke et al. 1999) and IMIRS package (Liang et al. 2002).
The main image processing steps are illustrated in Supporting Information, Figure S1.
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Screenshots depicting the main steps of image processing and analysis by Cioran and BRetina are shown in Figure 1.
As the main tasks of image processing, this paper introduces gray-level transformation, binarization, image filtering, image segmentation, visual object tracking, optical flow and image registration.
The raw data can be processed in different ways, and the past few years have seen the emergence of two main approaches: 2D image processing and 3D mesh processing algorithms.
The process of main works for conducting image processing is shown in Figure 1, and the descriptions are given as follows.
The main core of the image processing unit has been implemented by the differential algorithm procedure.
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