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All differentially expressed genes were input into MAS 3.0 and assigned individual GO terms for M. mulatta, with 53.15%, 30.58%, and 16.27% representing the main functional categories of biological process, molecular function, and cellular component, respectively).
Among the annotated transcripts exhibiting a down-regulation after challenging with the Fg don + strain, two main functional categories were over-represented (Additional file 2): (1) functions involved in photosynthesis (18 transcripts) and general metabolism, in particular glycolysis (4 transcripts).
These were further classified into 31 functional groups (GO term of level 2) according to three main functional categories: biological process; cellular component; and molecular function (Additional file 3).
We then conducted a GO analysis to convert the specific differentially expressed gene identifiers to standard and interoperable identifiers and to find the main functional categories in terms of biological processes, molecular functions, and cellular components.
They were significantly enriched for 132 GO terms over three main functional categories at p < 0.05, biological processes (BP) (93), molecular function (MF) (31) and cellular components (CC) (8).
Combination of the resulting functional annotations for all the genes in the list [see Additional File 6] allowed assignment of more than one biological function to certain genes and gave an overall classification in twelve main functional categories (Table 3).
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The highly enriched GO terms (p < 10−9) found in each main functional category is shown in Figure 4.
The main functional category affected in pyloric caeca of salmon after the substitution of dietary FO with VO was metabolism, which was not surprising bearing in mind the functions of this tissue.
A detailed analysis and annotation of the EST-SSRs revealed the locations of the polymorphisms within the transcripts, and that the main functional category was related to transcription and post-transcriptional regulation.
One of the main functional gene categories controlled by Nrg1p is related to peroxide tolerance, which is a specific trait of R57 [ 8].
Additional File 3, Table S3 lists the main functional GO categories of transcripts that were differentially expressed between the sexes with a stringency of ± 1.5-fold change in expression (P < 0.01).
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