Therefore, we are keeping the main figure using FDR, but have added a note in the text about the robustness of the result to variations of the analysis.
In Kirgizstan, Mr Kulov, now one of the main opposition figures, used to head the state security service and was at one time mayor of Bishkek before being put behind bars in 2000; and in Turkmenistan, Boris Shikhmuradov, a former ambassador to China and once considered a staunch supporter of Mr Niyazov, defected in 2001 and set up his own opposition group in Moscow.
They infiltrate and alter the three main figures (I will use Shakespeare's names for convenience) in shifting and wonderfully differentiated ways.
In more recent times, especially from the 18th century onward, Chinese influence became noticeable in the details of paintings, particularly in the freer but still balanced arrangement of the main figures and the use of Chinese-style landscapes as subsidiary decoration.
The authors are encouraged to restructure all their figures into a well-organized set of main figures with minimal use of supplementary figures.
X-radiography reveals sketched orthogonal lines on the underpainting, used to indicate where the horizontal and vertical axis should meet at the main figures' heads, and that he used a compass to sketch the spandrels.
A religious painter trained in the tradition draws in the outline, often using printed designs for the main figures.
(c ) Detection of negative control PLAs (GFP AMPKα1; as shown in main Figure 3c ) was achieved using anti-GFP antibody titrated by immunostaining transfected HEK293T cells expressing AMPKα1-GFP fusion protein.
(a ) Detection of AMPKα1 by immunostaining primary MEFs (as shown in main Figure 3b ) was achieved using an α1 isoform-specific immunofluorescence-grade antibody exhibiting no cross-reactivity with AMPKα2-GFP expressed in HEK293T cells.
Supplementary figure 1A is described in the text as a broadened FRET histogram in the presence of ATP, but the 7.2 nM derived from Supplementary figure 1B (and used in main Figure 1B) is discussed in the paragraph before, suggesting that this value is obtained in the absence of ATP.
Who were the main figures, and what makes them notable?
