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The WGCNA and DESeq2 analyses both identified two main expression patterns (i.e. genes that were differentially expressed in pollinated corollas and genes that were differentially expressed at 18 hap) when comparing corollas from pollinated and unpollinated flowers at the same developmental age.
Hierarchical clustering analyses showed that positively regulated genes fit into three main expression patterns.
Successive clustering with increasing cluster number reflected this finding showing first the main expression patterns and subsequently the minor patterns.
Endosperm exhibited three main expression patterns (F-H) at protein level, as shown in Figure 4.
Nine genes at the FDR cutoff (.049-.05) with low expression and fold-changes, and falling into the three main expression patterns, were selected for verification.
Identification of 117 differentially accumulated protein spots representing 82 unique proteins and five main expression patterns enabled a chronological description of wheat grain formation.
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However, Type II was the main expression pattern observed in wheat and in Ae. peregrina.
To identify the main temporal expression patterns in response to insulin stimulus, treated-minus-control expression profiles of selected genes were clustered using K means clustering based on Pearson correlation.
Thus, our analysis indicates that there are two main pros expression pattern subclasses among CB type I and OPC NB lineages (Fig. 7A).
To get a broader perspective on the function of differentially expressed genes, we analyzed for over-representation of gene ontology (GO) categories in each of the three main gene expression pattern clusters identified in figure 4A.
The main features of expression patterns that give a wide utility in bioinformatic studies are: the functional information associated [2], the high conservation of gene coexpression groups along evolution [3] and the high correlation of these groups with biomolecular pathways or reactions [4].
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main splicing patterns
main expression levels
main expression waves
main expression differences
main resistance patterns
main expression domains
main communication patterns
main modification patterns
main expression profiles
main causation patterns
main attenuation patterns
main dietary patterns
main histological patterns
main expression clusters
main migration patterns
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