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Sequence-independent uORFs allow for low-level translation of the downstream main coding region [ 61].
Therefore, a comparative approach was used to identify the main coding region.
In the first iteration, the longest conserved ORF was designated as the main coding region.
We hypothesize that this sub-optimal uORF sequence context (compared to optimal Kozak consensus [ 47] sequence for the main coding region) would allow for leaky scanning [ 48, 60] of the uORF, and preferential initiation at the downstream main coding region.
The longest ORF in at least three out of five cereals was considered the main coding region.
Therefore, these mRNAs may shift to lighter polysome fractions even if translation of the main coding region is increased.
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The circular and double-stranded mitochondrial genome is around 16 kilobase pairs long and can be further divided into two main regions: the coding region and the control region.
Tzanis et. al [ 15] developed a methodology for the prediction of the TIS, called MANTIS, with three main components: Consensus, Coding Region classification, and ATG Location.
We therefore constrained our main analysis to a partial coding region of the FU f to represent FUs (a-f), which form the wall structure of the cylindrical haemocyanin decamer, and a partial coding region of the FU g, which forms a relatively loosely connected collar structure inside the cylinder [ 21].
Each mtDNA genome can be divided into two main parts: the control and the coding region.
The comparative analysis of the nucleotide sequences of Oct4 orthologs in seven mammalian species demonstrated a high conservation of the genomic organization, coding region, and the main elements (minimal promoter, distal, and proximal enhancers) of Oct4 gene regulatory region.
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