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Thus, melanic P. maniculatus are homozygous for an allele with a large 125 kb deletion (aΔ125kb), which eliminates the main regulatory region, the noncoding exons 1A, 1B', 1C, 1C, and coding exon 2. To test whether this 125 kb deletion affects the abundance of Agouti transcript, we measured Agouti mRNA in the skin of P4 pups.
The tmem33sh443 allele is predicted to generate a premature stop codon within coding exon 4, located 53 nucleotides downstream of an exon junction.
We have created a new allele of Splotch by replacing the first coding exon with a gene encoding Cre recombinase.
Lower panel: The coding exon arrangement of the human aggrecan gene is depicted and the regions of the core protein encoded by each exon is indicated.
Its promoter lies within intron 44 and first coding exon is exon 51.
Homologous recombination resulted in the deletion of coding exon 2 of Tph1 and coding exons 1 and 2 of Tph2.
These would contain a 5'UTR with at least the untranslated exon -1 and the coding exon 1, but without any of the other coding exons.
The main regulatory element responsible for the expression of Sox2 in pluripotent stem cells is located ∼4 kb downstream of the single Sox2 coding exon and it is named SRR2 (Sikorska et al., 2008; Tomioka et al., 2002).
The first coding exon is missing from Meg10 and Meg8.
The next protein coding exon is shared by both variants.
Top, partial genomic map of the third Fz3 coding exon.
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