Although the main cellular proteins could be matched easily among the cell lines, the 2-DE protein maps differed considerably, pointing to the already known phenotypic differences among the lines.
Unexpectedly, the global analysis of gene functions reveals that two main cellular functions, protein metabolism and chromatin structure/transcriptional regulation, could be deregulated by v-ErbA.
Although in vitro and animal studies report that P-glycoprotein is not the main cellular transporter protein for efavirenz [ 18], several studies in African HIV patients reported association of genetic variation in ABCB1 with efavirenz plasma/intracellular concentrations and/or treatment outcome.
Expression and phosphorylation level of main cellular signaling proteins were determined by western blot analysis.
Neither did the expression pattern of the main cellular iron storage protein Ferritin, which usually binds free Fe2+ and thus prevents further ROS production and accumulation [36].
The UPS is the main route of cellular protein degradation.
In case of protein misfolding, the polyubiquitination at lysine 48 is a tag for the proteasome pathway, one of the main processes of cellular protein degradation [ 8].
These include transferrin, a precursor of serotransferrin, as well as transferrin receptor 1b, which is responsible for cellular uptake of metals from transferrin and a form of ferritin, the main cellular iron-binding protein.
Dear Editor, The unfolded protein response (UPR) constitutes the main cellular response to perturbed protein homeostasis.
