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The four main candidate loci for Daphnia fecundity (= total offspring production) include one additive locus and strong dominance in three loci, with each locus accounting for 9 to 15% of phenotypic variance.
Insight into the genetic mechanisms associated with the three main candidate loci in this study may also be gained by examining homologous genes and functions associated with the groups of loci that have previously been found by Hess et al. (2013) to be highly linked to these 3 SNPs.
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To confirm our findings, we genotyped the candidate loci in an independent set of 89 AMHs.
For all the outlier data, candidate loci will be annotated by gene ontology database and the functional categories enriched with candidate loci would be considered as potentially selected.
Genetic diversity estimates for candidate loci under directional selection was significantly reduced in comparison to candidate neutral loci, whilst candidate balancing selection loci demonstrated significantly higher levels of genetic diversity (Kruskal Wallis test, P < 0.05).
Potential candidate loci contained within our QTL regions were identified and analyzed.
I think Vancouver is the main candidate for now.
Joint testing of multiple candidate loci can also dramatically improve power, despite model misspecification and inclusion of irrelevant loci, but requires an a priori hypothesis defining the set of loci to investigate.
Various candidate loci under selection showed significant inter-chromosomal linkage disequilibrium, suggesting possible gene-networks underling adaptive phenotypes.
In total, we examined 95 candidate loci.
Finally, we used allozymes to test for candidate loci.
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