The tree (Figure 4A) has two main branches corresponding to two widely divergent clusters of sequences.
Supporting this, robust phylogenies (Figure 1) were observed with maximum bootstrap support (97 100%) in the three main branches corresponding to the traditional chlamydial serogroups.
The dendrogram has two main branches corresponding to entirely ACAT-related or entirely DGAT2-related sequences.
An unsupervised hierarchical clustering analysis identified two main branches corresponding to unstimulated and stimulated samples, indicating that cytokine treatment had the strongest effect in discriminating between samples, overcoming disease status (Fig 3A).
This tumor phylogenetic tree is equivalent to the one generated with the previous subset of 20 mutations, showing two main branches, one corresponding to the primary tumor (P1 region) and the other subdivided in two sub-branches which contain each of the recurrences samples (data not shown).
This dendrogram shows a clear discrimination among three main vertical branches corresponding to (i) Control, proliferating K-Raslox MEFs, (ii) the BRAF- or MEK1-rescued MEFs and (iii) the non-proliferating, Rasless cells.
This dendrogram allowed a clear discrimination of three main vertical branches corresponding to (i) non-proliferating Rasless cells as well as proliferating (ii) control K-Raslox MEFs and (iii) MEFs reverted to proliferate after transfection of Rasless cells with BRAF or MEK1.
Interestingly, the cluster analysis identified three main DNA methylation branches corresponding to cerebellum (granular cell layer and Purkinje cells), cerebral cortex (CX, CA1, CA3 and dentate gyrus) and diencephalon-basal ganglia (basolateral amygdala, caudate-putamen, substantia nigra, hypothalamus, globus pallidus and TH).
Bootstrap values of 1000 were obtained for all the main branches except for the one corresponding to the Class 3 APN.
Upon entering the Working Project, a maximally-collapsed CVTree with three branches, corresponding to the three main domains of life, appears as shown in Figure 1.
It is also puzzling that patients with a homogeneous pattern of increased F-FDG uptake in the aorta and its main branches and no arterial wall abnormalities on the corresponding CT slices are diagnosed as either TA or GCA, with only their age (≤ or ≥ 50 years) as the discriminating parameter [ 54, 55, 58, 60, 98, 108, 109].
