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Our results however paralleled a recent large-scale mRNA expression analysis on the three main berry tissues [32], as well as the skin proteome analysis of cultivar Cabernet Sauvignon where most of the proteins over-expressed at maturity were involved in pathogen response [8].
The three main berry tissues, seed or endocarp, flesh or mesocarp and skin or exocarp, were differentiated at the different developmental stages, and their dissection was only possible after certain stages.
These results paralleled a recent large-scale mRNA expression analysis on the three main berry tissues [ 17] as well as the skin proteome analysis of cultivar Cabernet Sauvignon where most of the proteins over-expressed at maturity were involved in pathogen response [ 24].
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Finally, the subcellular localizations of the enzymes within berry tissues were also investigated using immuno-gold electron microscopic technique.
Comparing EST frequency counts from cDNA libraries of mixed or water-deficit stressed leaf and berry tissues, respectively, with those from cDNA libraries from unstressed leaf and berry tissues, a total of 739 transcripts were identified and clustered into four main clusters.
It was also present in all berry tissues.
In most cases, transcript abundance was lowest in young berry growth stages and increased gradually until harvest in berry tissues.
In summary, different QTLs were identified for the same variables, depending on berry tissues.
However, no V-ATPase genes were found to be expressed differentially in berry tissues.
Overall, berry tissues were found to express approximately 76% of genes represented on the Vitis microarray.
Arrays of experimental conditions encompassing berry tissues and berry developmental series as well as limited stress conditions and management treatments were sufficiently represented in the public domain.
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