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Thus, comparing the magnitudes of expression changes in genes expressed at different levels or genes in different datasets requires knowledge of their individual signal detection slopes.
speA, speB and speC had similar magnitudes of expression in wild-type and csgD mutant cultures, whereas cadBA was slightly elevated in wild-type cultures (Figure 4, Table S1).
The magnitudes of expression of the ceoB and kdpA genes were pH-dependent being inversely related to decreasing extracellular pH.
We also identified genes that exhibited different magnitudes of expression change in response to infection in virgins vs. mated females using females that lack germlines.
speA, speB, speC and cadBA, the main genes encoding polyamine biosynthesis enzymes, had similar magnitudes of expression in wild-type and csgD mutant cells.
The magnitudes of expression of the sigA housekeeping gene, during early- and mid-log phases, were comparable between the two strains and were considerably higher than those of the K+-uptake encoding genes.
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However, in terms of the magnitude of expression, several genes strongly express the Apo-specific allele, the paternal allele for this specific hybrid cross, as shown by the high fold-differences between the two parental alleles.
We next looked at the magnitude of expression reduction of targets of highly expressed miRNAs compared to genome-wide background.
Both the number of differentially expressed genes and the magnitude of expression changes increase drastically along the time-course experiment (Table 2).
The existing data suggest that for relatively abundant transcripts the existence and direction (but not the magnitude) of expression changes can be reliably detected.
We conclude that maintenance of genetic stability which is achieved using mH5, though not with pSyn promoters, is linked to timing, not the magnitude of expression levels of foreign antigen, which is more closely associated with immunogenicity of the vaccine.
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