Sections were digitized at appropriate magnification and the areas of the dentate granule cell layer (GCL) were measured using ImageJ software.
Images were digitized at ×400 magnification, using a Leica DM2500 light microscope equipped with a Leica DFC420C digital camera, connected to a PC, with image resolution of 1728 × 1296 × 24 bits.
Immunohistochemically stained slides were digitized at × 20 magnification utilizing an Aperio Scanscope CS (Aperio, Vista, CA, USA).
All IHC cases were digitized utilizing at 20× magnification using an Aperio CS scanner (Vista, CA) and the images were reviewed in SlidePath software (Leica, Buffalo Grove, IL).
The selected areas were digitized by using a Sony CCD camera mounted on a LEICA Leitz DMRB microscope (Leica, Wetzlar, Germany) at ×100 magnification.
The radiographs were digitized on a measurement table (Hasselblad Engineering, Gothenburg, Sweden) using linear gauges and a video camera with ×16 magnification.
Pre- and post-treatment lateral cephalograms from both groups were digitized using the Dolphin Imaging (Dolphin Imaging, Chatsworth, CA, USA) software to allow for landmark identification and adjusting for magnification.
Cephalograms were digitized by one investigator (V.B).
Stone casts were digitized with a 3D scanner (3Shape, DK).
Films were digitized using a Canon scanner.
