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The magnification was checked at frequent intervals using a carbon graticule.
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The patterns in sections of the gels in appropriate magnification were checked and spots were added manually to the master gel to allow matching unique spots present in the individual gels.
Following polymerization, the region of interest was checked employing low-magnification lens; using the point of a sharp scalpel the areas of interest (GPe and GPi) were cut out.
Mycorrhizal colonisation (arbuscules, vesicles and internal hyphae) was checked under a compound microscope (Olympus B × 41) at 100 × magnification.
This could mean that many abnormalities were detected at low magnification, and many of them were checked at high magnification.
All teeth were checked at 40× magnification and X-rays images.
Five fields were checked at 200× magnification as follows: 0 (absent), 1 (<2 foci), 2 (2–4 foci), and 3 (>4 foci).
Cover slides were fixed with mounting media (DAKO, Glostrup, Denmark) and slides were checked under microscope at 100 × magnification.
After grinding, the samples were checked with a stereomicroscope at 40× magnification to ensure complete cementum removal.
Stained sections were checked for endophyte presence using a microscope at ×200 magnification.
Boundaries for each focal projection were checked for accuracy with the chartings that were carried out at higher magnification (10×; Fig. 1).
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