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Specimens were visualised by using an Olympus IX51 inverted microscope at ×100 magnification using the cellF Soft Imaging System (Olympus, Zoeterwoude, The Netherlands).
The slides were imaged at X40 magnification using the Nikon A1M confocal microscope and NIS elements acquisition software (Nikon, UK).
A new technique for image magnification using the theory of fractals is proposed.
Fluorescence was recorded using a confocal Zeiss LSM 710 microscope (×63 magnification), using the same laser intensities and detector gain for all slides in the experiment.
Cells were viewed on a Leica DMI6000 B at 63X or 100X magnification using the FITC, Cy3, CY5 and DAPI filter settings and imaged with a Hamamatsu Photometrics Delta Evolve camera.
Images were acquired at 20× magnification using the EVOS™ FL Imaging System (Thermo Fisher Scientific, AMF4300) or the Axio Oberserver.Z1 (Zeiss) of the Light Microscopy Facility, a core facility of BIOTEC/CRTD at the Technische Universität Dresden.
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Imaging is a vital tool in all areas of Drosophila research; routinely the tissue is dissected and imaged either at low-magnification using the stereomicroscope or at high-magnification using either compound or confocal microscopy.
At higher magnification using light microscopy, the nature of the immunostaining pattern was also characteristic with high density of individual MGL-immunoreactive puncta of varying sizes and intensities (Fig. 1E).
The total error of the measurement system was mainly due to the magnification used for the stereo-microscope and the size range of particles to be measured, being as low as ±5.7% for ×6 magnification and particles as small as 250μm.
The individual cells positive for cyclin D1 and Ki67, and total number of tumor cells were counted manually at 40× magnification using photographs taken from the individual tumor sections selected by the pathologist.
Figure 2 showed pictures under light microscopy with 400× magnification, using DAPI immunostaining of the nucleus.
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