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The 3D reconstruction on variable zoom shows that higher lens magnification results in a more accurate 3D sensing system.
The agreement between i-button sensor measuring results and image magnification results demonstrated the possibility of non-invasive measuring technology by image magnification.
Typically, an increase in magnification results in the electron beam having to be focused onto a smaller area in order to keep the number of electrons per pixel constant.
The system magnification results in a sampling density of 177 microns square geometry at the ocular pupil plane.
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The use of magnification resulted in images of extreme detail and clarity.
Sequentially tilted, energy-filtered (slit width 20 eV) images of individual cells were collected automatically using either the UCSF tomography [58] or Leginon software packages [59] in 2° increments up to +/−∼56 60° along either one (36 cells) or two (16 cells) axes [19] at 18,000× magnification, resulting in a CCD pixel size of 1.2 nm.
For the tissue samples, the optical magnification was set to ×10, vascular casts were scanned without binning with an optical magnification, resulting in a voxel size 0.7 μm.
All images were digitally recorded on a 2K × 2K CCD camera, at 14,500X or 19,000X magnification, resulting in a pixel size of 1.25 nm or 1.04 nm, respectively.
Measurements were performed with ×40 objective magnification which when added to the ×10 video ocular and ×2 internal magnification resulted in an image magnification of ×2500 on the monitor screen.
All immunopositive cells in the CC were counted in every 12th section in the right hemisphere in × 40 magnification, resulting in 5 7 sections per animal (n(6 h post IR)=7 9, n(4 months post IR)=9−15).
These metals are capable of bioaccumulation and bio-magnification, resulting to morbidity and often mortality in living organisms (Ayotunde et al. 2011; Ada et al. 2012).
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