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(A ) A high magnification phase image of the RPE layer of an USH2A eyecup prior to biopsy and subculture.
On day 2, 4, 7, 9, 11 and 13 after inoculation, we measured the density of paramecia by sampling 4 × 100 μl from each tube; ca. 60 individuals were fixed with lacto-aceto-orcein [ 41] to determine the proportion of infected individuals (at 1000× magnification, phase contrast).
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A glass cover slip was placed on the mixture, a drop of immersion oil added and the bacteria were inspected in a light microscope with 100× magnification, phase-contrast and a green filter.
Our tracing of growth cone outlines was performed on relatively low-magnification phase contrast (in vitro) or fluorescent (in vivo) images, and thus fine details of some filopodia were inevitably lost.
After 24 or 36 hours, cells were fixed with 4% paraformaldehyde in PBS for 5 minutes at RT and photographed by using a low-magnification phase-contrast microscope.
All cells were visualized at 40× magnification by phase contrast microscopy (Olympus DP70).
Slides were examined at 100× magnification under phase contrast to identify trypanosome forms and images were obtained using an Olympus CX41 microscope mounted camera (Olympus DP20).
Living cell cultures and stained cells were observed using a Leica DMLB inverted microscope with Hoffman contrast (LMC) (10× magnification) or phase contrast (20× magnification) and photographs were taken by a CoolSnap camera.
Planktonic cells of strains B. cereus G9241, B. cereus 10987, B. anthracis Sterne 34F2, and B. subtilis 168 (obtained from ATCC) were mounted on glass cover slips and hanging drop slides and observed under 40× magnification via phase contrast microscopy for motility.
To visually observe the cytopathic effect (CPE) that HSV-1 had on A549 and Vero cells and to determine if BTE could inhibit HSV-1, either by reducing or preventing the observable CPE, treated and untreated cells infected with HSV-1 were observed at 400X magnification using phase contrast microscopy.
The cells were observed at different magnifications through phase contrast microscopy (convolution).
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