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The tail fin of a Tg(CMV EGFP-map1lc3b) larva infeCMV EGFP-map1lc3bson larvaed bacterinfectedi) withfirst imaged using low magnification CLSM (Fig. 9A), followed by higher magnification imaging of the region of interest and 3D rendering (Fig. 9B).
The CHDM microscopy suites in our Carmichael building are fully equipped for whole-mount zebrafish imaging or high magnification imaging of sections.
High magnification imaging of the depot site showed individual particles dispersed within the cutaneous tissue.
Alexa-488 conjugated-secondary antibody (Invitrogen) was used for laser scanning confocal microscopy (Zeiss 510) (63X magnification) imaging of cytochrome c fluorescence staining in PMH.
Low magnification imaging of the unstained AIRs exhibited layers of wheat bran such as the aleurone (the outer layer of the endosperm) and the outer pericarp, together with the starchy endosperm.
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High-speed high-magnification imaging of the combustion is performed, and surface profiles and features of quenched sandwiches are investigated, besides burning rate measurements.
High-magnification imaging of the corrosion process from side view discloses that three different types of hydrogen bubbles are generated during corrosion: i) large and stable bubbles on the uncorroded regions, ii) a stream of fine bubbles at the corrosion front and iii) medium size bubbles behind the corrosion front.
High-magnification imaging of the Bacillus subtilis biofilms was performed at an operating voltage of 2 kV under an S-4500 Hitachi FESEM (Hitachi, Japan) at the MIMA2 platform.
Nicely preserved outer retinal architecture was observed with both methods, however, QFDE provided excellent high magnification imaging, with greater detail, of the apical, central, and basal planes of the RPE.
Higher magnification imaging also allows derivation of multiparametric outputs for characterizing complex phenotypes of spheroids treated with compounds.
Further, intact microparticles were observed by high magnification imaging within the cortical regions of the lymph nodes, indicating trafficking of particles by cells from the implantation site.
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