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To determine tumor cell proliferation and apoptosis, BrdU/TUNEL/or Ki67 positive nuclei were counted in randomly chosen 40× magnification fields of tumor tissue.
Five different high magnification fields of represented areas of tumours were evaluated.
To visualize low magnification fields of view of the mutants following pipetting, cells were pipetted rigorously to break up chain colonies and concentrated 30 100-fold by centrifugation.
Each condition consisted of two or more wells and two to four 200× magnification fields of view were captured per well.
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Disregarding, for the moment, any complex distortions or aberrations, the main differences between the two modes should be accounted for by some amounts for translation (detector position mismatch), rotation (detector orientation mismatch) and magnification (field of view mismatch).
Figure 4a shows a typical 40 × magnification field of the transwell unit membrane after removal of the nonmigratory cells.
Figure 2A,B,C, which represents a low magnification field of cells stained with the anti-P-Ser-HP1γ, demonstrates that the level and distribution of the signal for this modified form of HP1γ is variable in epithelial cells growing under normal conditions.
The motilities of human spermatozoa cells from random high magnification fields (100x) of the sample were determined in duplicate using atomic force microscope.
Three high magnification fields within the infarcted region of each section were chosen randomly.
Cells were photographed at × 10 magnification (four fields of view) and counted manually.
For each tumour, microvessels were counted within a 200 x magnification field in the area of highest microvessel density.
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