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The conodonts are studied under high magnification by using a binocular microscope.
Images were acquired at X200 magnification by using an Olympus BX53 microscope (Olympus, Center Valley, PA).
Images were captured at × 100 magnification by using a Zeiss Axiovert Fluorescence Microscope (Carl Zeiss, Gottingen, Germany).
Slides were scanned at 20× magnification by using an Aperio ScanScope Digital Slide Scanner (Aperio Technologies, Inc., Vista, CA).
Colonies were counted in five randomly selected fields at ×10 magnification by using Olympus IX50 inverted microscope.
The areas of tumor nodules were quantified in 8 representative images taken at 10 × magnification by using the imageJ software.
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The plots in Fig. 4 also justify the capacity of DHM for achieving a wide range of magnifications by using one MO.
JMJD2B positive cells were counted in ten random areas at 400× magnification and analyzed by using image J. Quantification of immunostaining assay is represented as percentage of JMJD2B positive cells.
Images were viewed at 40x magnification and captured by using a Nikon confocal microscope (Nikon D-ECLIPSE C1, Japan).
The ganglion, dissected together with the sympathetic trunk, was desheathed and pinned to the bottom of a chamber mounted on the stage of a compound microscope; individual neurons were identified at a magnification of ×500 by using diffraction interference optics.
The total number of Brn3a-positive cells in the retinas, located approximately at the same distance from the optic disk (7200 sq. microns, 40× magnification) was quantitated by using Scion Image analysis software (Scion Corp., Frederick, MD).
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