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(D) Tissue sections were visualized and photographed using an AxioCamMRm3-2 fluorescameracattachedtoched to a Zeiss Imager M2 fluorescence microscope at 400× magnification and software enlarged.
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Hoechst- and GFP-fluorescence were analysed using a Zeiss Fluorescence microscope (1000x magnification) and Axiovision software.
Nuclei were stained with DAPI (Sigma-Aldrich) before being observed with a LSM510 laser scanning confocal microscope (Carl Zeiss) at 40× magnification and LSM510 software was used for the analysis.
Sections were analyzed with a Zeiss Axioscope 2 (Carl Zeiss, Oberkochen, Germany) using objective lenses with 200× magnification and AxioVision software (Zeiss).
Images and EDS data were taken at ({times 60}) magnification, and Quantax 70 software was used to obtain the Ni and Co compositions from the EDS spectra.
Composition measurements were extracted from EDS spectra taken at ×250 magnification, and Quantax 70 software was used to extract Ni and Cu compositions from the spectra.
Images of invaded cells were acquired under microscope at 40× magnification using SPOT RT digital camera and software.
The bone area fraction occupancy (BAFO) between plateaus was determined at 100x magnification with the same microscope and software by subtracting the percentage area occupied by bone from the total available area within the healing chambers.
Migration of DNA strand breaks (comets) was visualised using an AxioImager fluorescence microscope at × 400 magnification and AxioVision v 4 software (Zeiss, Germany).
Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.
Sections were overlayed with ProLong Gold antifade reagent with DAPI (Invitrogen) and analyzed with a TissueFAXS System microscope at 100 200× magnification and the corresponding HistoQuest software (TissueGnostics).
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