Sentence examples for magnetic sorting using from inspiring English sources
The CD133− and CD133+ fractions were separated by magnetic sorting using the CD133 Microbead kit (Miltenyi Biotec, Auburn, CA).
Monocytes were isolated from buffycoats by Ficoll amidotrizoate density centrifugation and magnetic sorting using anti-CD14-coated beads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer's instructions.
GDTc were enriched from the PBMC fraction by magnetic sorting using the Miltenyi γδ T cell positive selection kit (Miltenyi Biotec, Auburn CA) as per the manufacturer's instructions.
Isolation of specifically desired cells was performed by magnetic sorting using the Miltenyi Biotec magnetic microbeads, MACS columns and MACS separator, according to the manufacturers instructions.
In some cases, total RNA was extracted from either CD4 or CD8 T-cells enriched by positive magnetic sorting using an AutoMACS (Milteny Biotec).
B cells were further isolated from these samples by magnetic sorting using CD19 labelled magnetic check beads (Milteny Biotech, Bergisch Gladbach, Germany).
Porcine immature DCs were differentiated in vitro from blood monocytes isolated from PBMCs by immune-magnetic sorting using the Anti-CD172 monoclonal antibody and treated during 3 4 days with a mixture of recombinant porcine granulocyte-macrophage colony stimulating factor and interleukin 4 [NCP and FL, [23].
The yeast library was subjected to two rounds of magnetic-sorting using biotinylated hGPC3530-558 or three rounds of magnetic-sorting using biotinylated hGPC3368-548-GST to enrich hGPC3-specific scFv-expressing yeast.
NKT cells were enriched from adult PBMC or neonatal CD4+ T cells by staining with Vα24 antibody, followed by goat anti-mouse magnetic beads (Miltenyi Biotec), and positively sorted by magnetic cell sorting using AutoMACS (Miltenyi Biotec) as described [30].
The gonads were dissociated with 0.05% trypsin/0.02% DNase I (10 15 min, 37 °C), and endogenous PGCs were removed by magnetic cell sorting using anti-SSEA1 antibody conjugated with magnetic beads (Miltenyi Biotec) according to the manufacturer's protocol.
Cells bound with hCD58-BV were isolated by magnetic cell sorting using a MACS system (Miltenyl Biotech).
