Sentence examples for magnetic separation protocol from inspiring English sources

CD8+ T cells were negatively selected from the obtained PBMC fraction by applying the magnetic separation protocol CD8+ T cell isolation kit II (depleting CD4, CD14, CD16, CD19, CD36, CD56, CD123, TCRγ/δ, and CD235a; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions.

Magnetic cell isolation was performed using the protocol for magnetic separation from Miltenyi Biotec.

The concentration of Con A conjugated to the silica-coated iron oxide nanoparticles was determined through the BCA assay, in accordance to the manufacturer's protocol (Pierce), after magnetic separation of the nanoparticle suspension using an MES buffer-equilibrated (0.1 M) LS25 MACS® column (Miltenyi Biotec).

Beads and buffer were then separated by magnetic separation and the supernatant was removed.

The cells were then washed and resuspended in 500 µl cold buffer, and magnetic separation was performed according to the manufacturer's protocol (Miltenyi Biotec, Germany).

The Eppendorf tube was then placed into the magnetic separator, and after a 1-min magnetic separation period, the supernatant was carefully removed without disturbing the beads.

TxB2 and PGE2 were determined by radioimmunoassay according to the manufacturer's protocol (Amersham Pharmacia Biotech Ltd ,UK) using the Amerlex-M Magnetic Separation System at the Department of Biochemistry, College of Medicine, Istanbul University.

After 45 minutes magnetic separation using microcolumns and the µMACS separator was performed.

To enable the expression profiling of individual leucocyte subsets we have developed and optimised a cell separation protocol based on sequential rounds of positive selection using magnetic beads.

Magnetic separation was performed as described in the magnetic-activated cell sorting (MACS) protocol (Miltenyi Biotec, Sydney, NSW, Australia).

Synovial macrophages were isolated by positive magnetic separation with CD14-conjugated magnetic beads (MACS; Miltenyi Biotech) and a magnetic cell separator (MACS) in accordance with the manufacturer's protocol.