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CD4-positive cells were isolated by placing the test tubes in a magnetic separation device (Dynal magnet).
Genomic DNA was further isolated using the Wizard® Magnetic 96 Plant System (Promega Corporation, Madison, WI, USA) and the MagnaBot® 96 Magnetic Separation Device, according to the manufacturer's instructions.
Our preliminary in vitro experiments demonstrate >95% first pass capture efficiency of magnetic spheres within a prototype high gradient magnetic separation device.
After washing (600 ×g, 15 minutes), cells were resuspended in 100 mL of PBS-HSA, counted, and characterized for immunophenotype and CD133+ endothelial progenitors were selected using the CliniMACS Magnetic Separation device (Miltenyi Biotec, GmbH, Germany).
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Magnetic beads with C8-functionality were divided into 5-μL aliquots in a 96-well microtiter plate, which was placed on the magnetic beads separation device (MPC-auto96, Dynal, Oslo, Norway) with the magnet down.
This paper investigates the feasibility of a cost effective high gradient magnetic separation based device for the detection and identification of malaria parasites in a blood sample.
After enrichment, a 1 mL aliquot of each sample type (fecal grab and PS) was subjected to immunomagnetic separation (IMS) using Dynabeads anti-O157 and a PickPen® magnetic particle separation device as per manufacturers' instructions.
To achieve a fully optimised magnetic classification within magnetic separation on a device, some steps must be followed.
Economical and practical advantages of using magnetic classification within magnetic separation on the same device applying only one step are described, as well as some potential industrial applications.
A WCX (weak cation exchange) magnetic bead kit (Bioyong SPE-C) and robotic separation device for a 96-well plate format magnetic separator were purchased from Boiyong Company.
A weak cation exchange (WCX) magnetic bead kit (Bioyong SPE-C) and robotic separation device for a 96-well plate format magnetic separator were purchased from Bioyong (Bioyong technologies Inc).
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