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We also evaluated the in vitro anticancer effects of these curcumin-loaded PEGylated magnetic liposomes using MTT assay.
MTT assay was conducted to determine the cellular cytotoxicity of liposomes and magnetic liposomes using L-929 cells, as the mammalian cell model.
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However, we found a similar solubility for E2 (˜4 mol%) and E4 (˜2 mol%) into palmitoyl-oleoyl-phosphatidylcholine (POPC) liposomes using nuclear magnetic resonance, indicating that their uptake is equivalent (Supplementary Fig S2A).
Liposomes used were large unilamellar vesicles.
This chapter reviews the different cancer detection and treatment modalities using magnetic liposomes and their advantages over conventional approaches.
For the assays the liposomes were diluted into external transport buffer in a 0.85-ml micro cuvette with a small magnetic flea (5 mM HEPES pH 6.8 or 5 mM MES pH 6.0, 2 mM MgSO4 and the desired amount of KCl to obtain the desired potassium gradient, ionic strength was kept equal across the liposome using NaCl).
For CAMNP and DOX-loaded magnetic liposomes, TEM was conducted without using PTA.
The magnetic liposomes were then isolated at 25 °C using a magnet column where PBS (pH 7.2, 10 mM) with 50 mM NaCl served as an isocratic mobile phase.
For OAMNP and curcumin-loaded PEGylated magnetic liposomes, TEM imaging was conducted without using PTA.
In this study, we developed the cancer treatment through the combination of chemotherapy and thermotherapy using doxorubicin-loaded magnetic liposomes.
When the magnetic liposomes with 1 μM doxorubicin was used to treat CT-26 cells in combination with HFMF exposure, approximately 56% cells were killed and found to be more effective than either hyperthermia or chemotherapy treatment individually.
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