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Monocytes were isolated by an indirect magnetic labelling method allowing isolation of untouched monocytes from human peripheral blood (Miltenyi Biotec, Bergish Gladbach, Germany).
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Here we describe an instantly cell magnetic labeling method based on ultrasound.
We describe the applications of new cellular magnetic labeling method to endothelial progenitor cells (EPC), which have therapeutic potential for revascularization.
CD4+ and CD8+ T cells were isolated by indirect magnetic labeling method (Miltenyi Biotec, Friedrich-Elbert, Germany) using the manufacturer's protocol.
Several magnetic cell labeling methods have been developed but the most commonly used one is coincubating Dextran-coated nanoparticles with a transfection agent [ 48].
There are a wide range of labels and methods for attaching these to polymers, and the resulting fluorescence behaviour can depend on both the label and labelling method.
pDCs were obtained from PBMCs by using MACS magnetic labelling system (Miltenyi Biotec, Bergish Gladbach, Germany).
Monocytes were sorted from PBMC using indirect magnetic labelling based on the expression of CD14.
But the labelling method needs some discussion.
Direct or transient labelling methods, such as iron-labelled cells for magnetic resonance imaging (MRI) or 111In-oxine-labelled cells for single photon emission computed tomography (SPECT) are unsuitable for the time frame of immunotherapy studies.
The use of magnetic labeling of cells and an external magnet has been proposed as a minimally invasive method to deliver injected MSCs to defects.
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