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We used a magnetic gravity column isolation to obtain quiescent human peripheral blood TH2 cells.
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TH2 lymphocytes were negatively isolated from the PBMCs using StemSep™ magnetic gravity columns (StemCell Technologies, Vancouver, BC) with a monoclonal antibody cocktail we previously validated for TH2 enrichment to 84% purity[ 23].
Magnetic, gravity, and subglacial topography data reveal a tectonic boundary within East Antarctica.
The supernatant was loaded onto a gravity column of Ni2+ affinity resin (Ni-NTA, Qiagen).
A gravity compensator has been realized in a test setup that shows the feasibility of the chosen modeling technique and of magnetic gravity compensation.
10 ml serum was run over a gravity column packed with 0.5 ml beads.
The supernatant was applied to Ni-NTA resin gravity column (Qiagen) that had been previously equilibrated with PBS.
EAE was fractionated using gravity column chromatography.
Analytical grade chloroform and methanol were used for gravity column chromatography.
The proteins were loaded on a small gravity column containing 0.2 ml Ni-NTA resin.
Silica gel 60 G 0.063-0.200 Merckerck; Germany) was used for gravity column chromatography.
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