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SCA-1+ cells were positively selected using the MACS® magnetic bead system (Miltenyi).

B cells were purified with a CD19+ magnetic bead system (MidiMACS, Miltenyi Biotec, Bergish Gladbash, Germany), according to the manufacturer's instructions.

Immunoprecipitation (IP) of cystatin C from CSF was performed using a magnetic bead system (MACS Molecular, Auburn, CA).

Aliquots of PCR products from HPV positive samples were then re-arrayed into 96-well plates and purified by the AMPure magnetic bead system (Agencourt Bioscience Corporation, Beverly, Massachusetts, USA).

Sequenced PCR product was purified using AMPure magnetic bead system (Agencourt: Morrisville, NC) following manufacturer's protocol, analyzed on an ABI 3730xl DNA Analyzer (Applied Biosystems International), and edited with Sequencher version 4.7 (Gene Codes: Ann Arbor, MI).

Similar(55)

CD19+ and CD19− cell populations were collected using the magnetic beads system described earlier (see Materials and Methods).

However, immune-magnetic bead system is susceptible to sample loss due to multi-step sample preparation.

Then, with the magnetic bead isolated system and target DNA-induced hybridization chain reaction (HCR), we realized the specific target DNA analysis with a low detection of 0.228 nM, and demonstrated its effectivity in distinguishing the target DNA from other interferences.

DN γδ T cells were purified by negative selection from the LNs of WT γδTCR Tg, Lck+/− γδTCR Tg and Fyn+/− γδTCR Tg mice using the MACS magnetic bead separation system (Miltenyi Biotec, Auburn, CA, USA).

The population of Treg was depleted using two different methods: in three subjects, we removed the CD4+CD25+ Treg using a magnetic bead separation system (AutoMACS cell separation, Miltenyi Biotec), as per manufacturer's instructions, while in the other 4 subjects, we used a flow-based cell sorter (Beckman Coulter MoFlo) to separate the CD4+CD25hi T cells.

When the physical nature of the milk samples was too altered, DNA purification with a magnetic bead-based system was used.

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