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All PCR products were cleaned using AmPure® magnetic bead solution (AgenCourt, Beverly, MA) and rehydrated with deionized, double-distilled water.
The supernatant was discarded and the precipitate was washed twice with TBS solution that was equivalent to 20-fold volumes of the magnetic bead solution.
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Briefly, 3 μl each of single-cell lysate containing oligo-dT magnetic beads, washing solution 1, washing solution 2, reverse transcription solution, washing solution 3, terminal transferase solution, washing solution 4 and PCR solution were dispensed onto the fabricated film of the MAGrahd reactor tray.
The mixture was agitated at room temperature for 10 min and then placed in a magnetic rack to separate the magnetic beads from solution.
Exon capture involves the hybridization of genomic libraries to short oligonucleotide baits complementary to complete or partial exomes printed on a microarray [ 7] or attached to magnetic beads in solution [ 11].
In order to elute the RNA, the beads were resuspended in 15 μL of elution buffer (20 mM Tris-HCl, pH 7.5, and 1 mM EDTA), incubated for 3 min in 95 °C, and placed in a magnetic rack to separate the magnetic beads from solution.
Copper-chelated magnetic beads and solutions were used for extracting peptides from serum samples [ 25].
After final washes with double distilled water, a further 45 µL of water was added and the tube heated to 90°C for 2 min to elute captured DNA before placing the tube immediately on a magnet with aspiration of the supernatant as soon as the magnetic beads cleared from solution.
PCR fragments were purified using magnetic beads and SPRI solution.
The final concentration of the bead solution is 0.1% carboxyl-modified Sera-Mag Magnetic SpeedBeads, 18% PEG-8000 (w/v), 1 M NaCl, 10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0), and 0.05% Tween 20.
Based on the principle of magnetic bead adsorption, magnetic beads were released through special magnet adsorption to accomplish the shift between magnetic beads and nucleic acids.
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