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Plasmid DNA was purified from individual subclones by an automated magnetic bead process (Agencourt Bioscience).
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Magnetic bead processing on an automated platform makes the process as described here well-suited for moderate to high sample numbers, and further automation is possible.
One tube was processed according to the prototype magnetic bead processing protocol (Microsens Medtech Ltd., London, UK).
A novel and highly sensitive electrochemiluminescence (ECL) assay based on magnetic beads separation/collection process and thymine Hg2+ thymine (T–Hg2+–T) coordination chemistry has been designed to detect Hg2+ ions in aqueous solution.
Thus, phages with strong affinities and slow off-rates could remain on the magnetic bead surface during the washing process.
The globin mRNA depleted RNA was transferred to a fresh tube and further purified with a rapid magnetic bead-based purification process.
Based on the principle of magnetic bead adsorption, magnetic beads were released through special magnet adsorption to accomplish the shift between magnetic beads and nucleic acids.
In an attempt to overcome the limitations of conventional LCR, a process called enzyme-free click chemical ligation reaction (CCLR) that involves Au NP and magnetic bead was developed by Kato and colleagues (Fig. 5A).
The first of these is immunity magnetic bead (IMB) technology.
CD14+ monocytes were isolated using magnetic bead separation with anti-CD14 antibody-labeled magnetic beads and iron-based MidimacsLS colums (MiltenyiBiotec).
One aliquot was purified by dextran sedimentation while the other was purified by magnetic bead depletion (magnetic beads coated with antibodies to CD4 and CD8).
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