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The cells were selected using a magnetic affinity column according to the manufacturer's recommendation.
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To identify autoantigens expressed by platelet MPs, we isolated the CD41+ mpICs from RA SF using magnetic affinity columns.
The key interactor is immobilized onto a solid support, such as a (spin) affinity column or (magnetic) beads.
The Leu-2 molecule was effectively purified from supernatant with an anti-Leu-2a affinity column.
POC1B312 406-His was POC1B312 406-Hisel beads, and POC1B312–406-GST was purified by glutathionickeld affinity column.
The supernatant was applied to a nickel affinity column (Qiagen) which was equilibrated with lysis buffer.
An affinity column with AAAA as the ligand was prepared.
This LC/MS system uses a column-switching system equipped with 2 columns, a protein A affinity column and a reversed-phase column (desalting column).
The supernatant was carefully collected and loaded to a pre-equilibrated 5 mL HisTrap Ni2+-NTA affinity column (GE Healthcare).
Sigel, E., Mamalaki, C. & Barnard, E. A. Isolation of a GABA receptor from bovine brain using a benzodiazepine affinity column.
These antibodies were purified by antigen affinity column.
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