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This gene was also not upregulated under starvation with other essential nutrients such as nitrogen, calcium and magnesium (data not shown).
No change in fluorescence was seen in the absence of magnesium (data not shown) confirming that nucleotide binding, as for other Arf GTPases, is Mg2+‐dependent.
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Results for dietary magnesium intake were similar to those observed for total magnesium intake (data not shown).
In addition, similar non-significant associations were seen when we analysed baseline magnesium intake (data not shown).
The diffractograms of SE powders and their solid components confirm the crystallinity of ibuprofen and the amorphous form of both magnesium aluminometasilicates (data not shown here).
In addition, both the presence of excess proteins (either whole serum or purified proteins like fetuin-A or albumin) and certain competing ions (magnesium or carbonate, data not shown; see also ref. [2]) are now known to result in smaller particle sizes.
We did not obtain detectable levels of de novo initiation using magnesium as divalent cation (data not shown).
In addition, generally non-significant associations were seen when we modelled magnesium intake as quintiles (data not shown).
Adjusting for the use of calcium channel blockers at baseline did not alter the harmful effect of magnesium in our study (data not shown).
In the control STH2370 ΔSPI-3::aph strain, a unique finding was the inability to grow at a low magnesium concentration (10 µM Mg2+) (data not shown).
However, there is a strict requirement for magnesium ions since the ADP dissociation constant increased to >5 mM, when magnesium ions were removed by EDTA (data not shown).
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