Exact(6)
In addition, there are indications of genetic toxicity to Salmonella[12] and acute toxicity to Daphnia magna with a LOEC of 100 mg L-1 after 24 h [5].
A sterile kaolin suspension, 0.02 ml (250 mg/ml in distilled water) was injected into the cistern magna with a 27-gauge needle.
Cerebrospinal fluid was obtained by puncture of the cisterna magna with a 26 gauge injection needle via the suboccipital access after the pentobarbital injection was given.
The microsporidian parasite Hamiltosporidium tvärminnensis exhibits pronounced seasonality in natural rock pool populations of its host, Daphnia magna with a regular increase of prevalence during summer and a decrease during winter.
(in 10 μL phosphate buffered saline (PBS); percutaneously into the cisterna magna with a 30-gauge needle in 45° anteflexion of the head 10) or systemically (i.p .. Anti-CD20 and ragweed isotype control were kindly provided by Genentech Inc (South San Francisco, CA).
hUCB-MSCs were stereotactically inoculated into the hippocampal area (AP: −2.3, L: ±1.3, DV: −2.0 referenced to the bregma) or the cisterna magna with a sterile Hamilton syringe fitted with a 26-gauge needle (Hamilton Company, Reno, NV, USA), using a microinfusion pump (KD Scientific, Holliston, MA, USA) in a stereotaxic apparatus.
Similar(54)
The first one shows acute toxicity to Daphnia magna with an LC50 of 0.23 mg L-1[17] and is also 'very toxic to aquatic life with long lasting effects' [5].
Asghari et al. [24] described the effect of AgNPs on the immobilization of Daphnia magna with an EC50 value around 47-fold lower, compared to the EC50 value of Ag+.
Other abnormalities, such as mild enlargement of the cisterna magna with an intact vermis and without other structural abnormalities or mass effect, punctate cerebellar haemorrhage (<4 mm), and small subdural or subarachnoid haemorrhage without mass effect, were considered minor and less relevant, based on available literature [ 16– 16].
The toxicity of γ-butyrolactone in zebrafish has not been investigated previously; however, unpublished data indicate acute (48 hr) toxicity in fish (golden orfe Leuciscus idus) with LC50 of 275 302 mg/L and in the daphnid Daphnia magna with an LC50 of > 500 mg/L (Lyondell Chemical Company 2004).
Digestion of P. magna mtDNA with a single-cutter (and two-cutter) restriction endonuclease, followed by PFGE and Southern blot analyses, gave sub-genome-sized bands, which migrated in accordance with the mitochondrial restriction map (supplementary fig. S1, Supplementary Material online); no genome-sized or high-molecular-weight bands or smears were observed.
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