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The same observation was made with phosphate.
Most H-bonds (53.3%) are made with phosphate groups of the DNA at the protein∶DNA interfaces.
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The dilutions were made with phosphate-buffered saline, pH 7.4, containing 1% bovine serum albumin.
Further dilutions were made with phosphate-buffered saline (PBS) supplemented with 0.1% bovine serum albumin, fraction V (BSA; Sigma Chemical Co., MO USA) [ 38].
Isotonic Percoll solution (IPS) was made with 10X phosphate buffered saline (PBS) pH 7.4 and Percoll (Pharmacia, Uppsala, Sweden) (9 1 v/v).
The tougher challenge is that all lithium-ion batteries are expensive, whether made with cobalt or phosphate.
Batteries made with lithium iron phosphate (LiFePO4) are a possible replacement, because that combo is considerably cheaper.
To investigate the lipid production by T. fermentans in the presence of 30 mM [Ch][OAc] with pH control, fermentation medium was made with 20 mM phosphate buffer, pH 5.0, 6.5, and 7.5, instead of water.
All solutions were made with 0.1 M potassium phosphate buffer pH 7.0.
Parallel comparison of the cellular behaviors of hASCs on the akermanite was made with those on beta-tricalcium phosphate (β-TCP).
The shell can also be made with inert materials such as calcium phosphate of apatite.
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