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Hence, this paper presents a testing method in which most of the influences were quantified or made variant while the support condition was kept invariant.
UnifiedGenotyper was given the target capture intervals of each experiment and only made variant calls in these intervals when calling INDEL and SNP variants.
We first discarded alignments with the mapping quality lower than 30, and then made variant calls by mpileup and bcftools programs embedded in SAMtools [ 20].
The net result of simultaneously having more false negative alignments and less false positive alignments seemed to have made variant calling easier and led to the lower false negative variant calls.
Intel, which loves to make its own chips, tapped TSMC, which loves to make chips for other companies, to make variants of its Atom processor.
Just as if they were making antibodies, the B cells mutated the RFP gene to make variants.
pDIMC8-RG13 was used as a template plasmid DNA to make variants using the PCR-based QuikChange mutagenesis procedure by Agilent Technologies.
Try making variants that involve vehicles.
Variant M1 retained high specific activity (993 U/mg; equivalent to 93% of wild-type activity) and improved thermal resistance (T50 improved by 1.5 °C compared to wild-type at 58 °C; 20 min incubation time), making variant M1 an attractive enzyme for industrial applications.
SNVer estimated the empirical error rate for those selected reads in making variant calls.
The additional coverage and diversity of GENCODE Comprehensive transcripts leads to the identification of many more genic variants than RefSeq, however, transcriptional complexity can also make variant interpretation more difficult (see Additional file 14: Figure S9).
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