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Exact(6)
Serial dilutions of up to 10−4 were made using sterile 0.85% (w/v) NaCl.
As described by Cappucino and Sherman (2002) 10−1 10−3 dilutions were made using sterile PBS solutions.
At the laboratory, the serial dilutions were made using sterile diluents, i.e. 10−1, 10−2 and 10−3.
All stock solutions were made using sterile solvents or endotoxin-free water.
Deposits were made using sterile filter papers saturated with the 10% tracer compounds (w/v), shaped to cover the appropriate cortical region, and inserted beneath the dura mater (Rubio-Garrido et al., 2007).
Spore suspensions were made using sterile distilled water containing 0.01% Tween-20 (vol/vol) and then filtered through a 100-μm cell strainer (BD Biosciences, Bedford, MA) to remove any debris and clumps of urediniospores.
Similar(54)
Then, bores (3 mm depth, 5 mm diameter) were made using a sterile borer and loaded with a concentration of 5 mg/mL of all samples.
A straight scratch injury was made using a sterile 1 mL pipette tip on 6-well plates.
After cells were more than 80% confluent in 6-well plates, the scratch was made using a sterile pipette and then treated with vehicle (DMSO) or compounds.
Bacterial quantifications for homogenized liquid were made using serial 10 fold dilutions in the sterile salt solution (NaCl 0.85%), followed by plating on an agar medium.
All aqueous solutions were made using diethylpyrocarbonate (DEPC) treated milli-Q water and routinely filtered through 0.22 μm sterile filtration systems (Millipore, Billerica, MA).
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