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Insulin infusion adjustments were made using sliding scales [10].
Insulin infusion adjustments were made using sliding scales [ 10].
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The estimation of these quantities was made using a sliding window of three consecutive reports.
The design of the custom array slide was made using the Agilent website dedicated to this purpose [ 15].
For this, microarray slides were made using over 7000 ESTs that we previously identified from normal and regenerating intestine cDNA libraries [ 13].
Detailed overview images of each stained slide were made using a Colorview IIIU digital camera (Olympus, Zeist, the Netherlands) mounted to a BX-40 microscope (Olympus, Zeist, the Netherlands).
An individual was considered negative for S. mansoni, all or any of the STHs species if no ova were observed in slide preparations made using both techniques.
In particular, the diagnosis should be made using the hematoxylin and eosin stained slide and no immunohistochemistry should be needed to arrive to the diagnosis.
Coronal sections (30 μm) were made using a cryostat and mounted on glass slides (Matsunami, Japan).
Dissections were made using tungsten needles, and appendages were mounted on microscope slides.
Slides of 5 μm in thickness were made using a microtome.
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