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✓ Morphological evaluation: Cytokeratin staining was made using mouse anti-human cytokeratin pan KL1, dilution 1/40 (Clinisciences, Montrouge, France) and vimentin staining by using mouse anti-human vimentin V9, dilution 1/200 (Biogenex, France).
Immunoblotting (7.5% SDS-PAGE) was made using mouse anti-NFATc2 (Santa Cruz Biotechnology, Santa Cruz, CA) and anti-mouse-IgG peroxidase-conjugate (Sigma Aldrich Taufkirchen, Germany) or goat-anti mouse IgG1-IRDye 800 (LI-COR, Lincoln, Nebraska, USA).
Previous observations made using mouse animal models suggest that MCP-1 and chemokine receptor 2 might be involved in the etiology of AMD and that macrophage dysfunction may have a central role in the early stage of AMD development [37], [38].
To study the molecular pathogenesis of microcephaly induced by DDR defects in humans, attempts have been made using mouse models with disruption of NBS1 (known as Nbn in mice) and ATR.
Although major progress has been made using mouse models to examine the disease progression (7– 10), our understanding is currently restricted by a lack of knowledge about the functions of, and even the precise location of, the CLN3 protein within cells.
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Mutant Pnkd transgene mice were made using a mouse BAC carrying A7V and A9V mutations in the mouse Pnkd ortholog; Pnkd null mice were made by deleting exons 5 9 of the mouse Pnkd ortholog.
Here, we aim to highlight the recent advances made using transgenic mouse models towards the understanding of the regulation of NCC and ENaC function relevant to the control of sodium balance and blood pressure.
Second, all responses had to be made using the mouse (instead of the keyboard).
A retrovirus bearing Bmi-1 was made using a mouse stem cell virus (MSCV) vector, which contains an internal ribosome entry site (IRES) followed by GFP (Figure 2A) [14].
Bmi-1 bearing retrovirus was made using a mouse stem cell virus (MSCV) vector MSCV-GFP-Bmi-1 (kindly provided by Dr. Morrison, University of Michigan), which contains an internal ribosome entry site (IRES) followed by GFP [14].
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CEO of Professional Science Editing for Scientists @ prosciediting.com