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Efforts were made to express ERS1 under an inducible promoter to obtain stable expression lines; however, the resulting siblings of any homozygous transformation lines exhibited various degrees of elevation in growth inhibition, possibly due to unstable expression (data not shown).
Previously, an attempt was made to express the G. stearothermophilus derived α-amylase SR74 gene in bacterial expression system (E. coli) which resulted in an amylase production of 15.3 U mL−1.
He had an acute sensitivity to the ways in which a building or landscape can be made to express a sense of the lives which are conducted there.
Andronico and Asteria are in love, so both Andronico's dutiful presentation of Tamerlano's proposal, and Asteria's apparent acceptance (so she can get close enough to kill him) lead to recriminations of the sort that flighty arias are made to express.
They say that a cluster of fl flowers can be made to express any sentiment; list of the various meanings and some interpretations.. View Article By Miranda Carter By Amos Barshad By Doreen St. Félix By George Packer.
Michelle Rodriguez has responded to vehement criticism of her new film [re]Assignment, by defending it as a "culture shock pic" she made to "express my frustration" with the current state of the film industry.
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States also use UN decision-making to express and foster support to the Court and to the fight against impunity.
In recent years, various efforts have been made to explicitly express the local stationary models.
Doubly transformed E. coli were made to concomitantly express the Prb library-NspGFP fusion and gankyrin-CspGFP fusion proteins, and incubated at 30 °C for 6 h.
Initial efforts have been made to generate expressed sequence tags (ESTs) from in vitro tissues of W. somnifera [ 30].
HEK293T cells were made to stably express LRP6-EGFP by transfecting pCS2+LRP-EGFP and pPUR (Clontech) into cells and treating with 2 mg/ml of puroMYCin.
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