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As mentioned above, no attempt was made to annotate missing ORFs.
Attempts were made to annotate these genes further using protein BLAST and HMMER but only uncharacterised proteins were hit.
Considerable effort has been made to annotate these ESTs and associate them with Gene Ontology (GO) terms to facilitate subsequent microarray analyses.
Though attempts have been made to annotate enhancers [ 20, 21, 40- 42], the general impression is that enhancers remain poorly annotated in the human genome.
Efforts have been made to annotate the non-coding regions of the genome [ 74], and in a recent study of 29 mammalian genomes, 3.5% of the non-coding regions were shown to be under purifying selection, indicating possible regulatory roles in the genomes [ 75].
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Few attempts have been made to systematically annotate protein domains that are largely made up of intrinsic disorder.
Molecular genetics is exemplary in that regard; the massive investments made to sequence and annotate the human genome and to refine the analysis of genetic information were only possible because of the idea that medical benefits will emerge down the line.
Four dTALE genes were made to induce the four annotated Xa27 homologous genes in rice cultivar Nipponbare, but none of the four induced Xa27-like genes conferred resistance to the dTALE-containing Xoo strains.
We have made no attempt to annotate uORFs, except in cases where they show evidence of encoding conserved polypeptides (Lin et al. 2007; Hayden and Bosco 2008).
Comprehensive catalogs of genes can be generated, annotated, and comparisons made to other genomes.
Further, joining of overlapping reads that were initially non-annotated made it possible to annotate an additional 1-21-2% totalotal reads.
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CEO of Professional Science Editing for Scientists @ prosciediting.com