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The overexpression of the threonine synthesis pathway and threonine dehydrogenase made the recombinant Halomonas TD08 able to produce poly 3-hydroxybutyrate-co-3-hydroxyvalerate) or poly 3-hydroxybutyrate-co-3-hydroxyvaleratete or 3HV, from various carbohydrates as the sole carbon source.
Moreover, it is one of the most xylose tolerant enzymes and this quality has made the recombinant enzyme very useful for saccharification of xylan-containing polysaccharides.
To test this idea, we made the recombinant adenovirus expressing green fluorescent protein-tagged Brn2 (GFP-Brn2), and transduced cultured human epidermal keratinocytes.
Glu at position 705 in the BPSM BvgS made the recombinant strain more responsive to nicotinate than its parent, with 4 mM nicotinic acid causing the complete loss of BvgS activity (Fig. 5B).
The replacement of both VFT1 and VFT2 of the BPSM BvgS by those of RB50 (BPBb1+2) did not affect the level of BvgS activity in the absence of modulation, but it made the recombinant bacteria much more sensitive to nicotinic acid than their parent (Fig. 4B,C).
TC made the recombinant SVec plasmids carrying the FTM- genes and established the expression system.
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The biochemical, immunological, and functional activities of C-rmCRP were shown to be comparable to biological mCRP, making the recombinant protein an excellent and advantageous research reagent for the study of mCRP.
It has recently been engineered to glycosylate proteins in a human-like manner, making the recombinant products more acceptable to the regulatory authorities [1].
Truncation makes the recombinant proteins soluble and more readily amenable to purification and structural characterization.
However, RD-1 substitution in BCG makes the recombinant strain gain virulence, and thus is not suitable for vaccine implication [ 10, 25- 27].
The ease of production of influenza virus-based immunogens and the availability of different vaccine formulations make the recombinant influenza viruses an effective vaccine platform for the delivery of foreign antigens and thus potentially suitable for heterologous prime-boost regimens.
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