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After all, Cuba totally made normalized diplomacy in-style this year.
For each synonymous and non-synonymous nucleotide substitution site, four measurements were made: normalized expected (ES and EN) and observed numbers (NS and NN).
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Single stranded transcripts and their homologs that remained in the treated reactions were PCR amplified to make normalized ds-cDNA.
For normalization, 300 ng of cDNAs were denatured, allowed to self-anneal, DSN treated (Duplex/double stranded specific Nuclease; Evrogen, Russia), and remaining transcripts were PCR amplified to make normalized ds-cDNAs.
Scatter plots were made from normalized signals.
The individual clones from the enriched in vivo cDNA libraries that only hybridized to the probe made from normalized in vivo cDNAs and the individual clones from the enriched in vitro cDNA libraries that only hybridized to the probe made from normalized in vitro cDNAs were chosen for sequence analyses.
Statistical comparisons were made on normalized Ct-values.
For 454 sequencing, we made a normalized cDNA library and a 3' cDNA library in cooperation with Takara Bio (Otsu, Shiga, Japan).
The individual clones that only hybridized to the probe made from normalized in vivo cDNAs were chosen for sequence analysis.
ESTs libraries representing unnormalized sequences were prepared as described in Taliercio et al. [ 51] and a comprehensive list of tissues represented is shown in Table 2. Vertis Biotechnologie (Freising-Weihenstephan, Germany) made the normalized libraries.
The obtained values for objective function will be used to make two normalized measures presented in Eqs.
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