Sentence examples for made mutations in from inspiring English sources

We made mutations in the N-terminal SH3 domain (W36K), C-terminal SH3 domain (W193K) SH2 domain (R86K), or both SH3 domains (dSH3m) using site-directed mutagenesis and added an HA tag (Fig. 4C).

We also made mutations in sptC, homologous to the yeast SAGA component gene SPT3, which showed a similar phenotype.

To test this idea, we made mutations in several putative DDK and CDK sites found in the vicinity of the RVxF and SILK motifs in budding yeast Rif1.

To test whether this interface is indeed biologically significant in the autophosphorylation process, we made mutations in key interfacial residues, Q636A and F637A, and determined their ability to autophosphorylate as compared with wild type.

To verify the functionality of NLS3 and NLS4, we made mutations in NLS3 (R353A, R353A, K360A, and R362A) or NLS4 (R494A, R494andnd K501A) in the context of full length p68.

To test whether mutations that interfered with the binding of glycogen to the GBD also affected inhibition of AMPK by glycogen, we made mutations in full-length β1 coexpressed with α1 and γ1 in CCL13 cells, isolated the complex by immunoprecipitation via the myc tag on α1, and assayed the kinase activity in the presence and absence of glycogen.

They can dispatch many probes at once and simply make mutations in the genes they want to study.

If ferrets mimicked humans and swine strains did not move between them,  researchers could make mutations in the viruses to determine the genetic changes that controlled the transmission.

By making mutations in bacterial DNA and examining the resulting phenotypes, scientists can determine the function of genes, enzymes and metabolic pathways in bacteria, then apply this knowledge to more complex organisms.

It is not yet possible to make mutations in P. carbinolicus.

We chose to further investigate the parallels between NOD1/2 activation by making mutations in NOD1 corresponding to a broader range of eight different BS/EOS SNPS.